The protective roles of TiO₂ nanoparticles against UV-B toxicity in Daphnia magna

Aquatic environments are increasingly under environmental stress due to ultraviolet (UV) radiation and potential inputs of nanoparticles with intense application of nanotechnology. In this study, we investigated the interaction between UV-B radiation and titanium nanoparticles (TiO₂-NPs) in a model freshwater cladoceran Daphnia magna. UV-B toxicity to Daphnia magna was examined when the daphnids were exposed to a range of TiO₂-NPs concentrations with an initial 5 or 10 min of 200 μW/cm² UV-B radiation. In addition, UV-B toxicity was also examined in the presence of TiO₂-NPs in the body of daphnids. Our results demonstrated that the daphnid mortality under UV-B radiation decreased significantly in the presence of TiO₂-NPs both in the water and in the body, indicating that TiO₂-NPs had some protective effects on D. magna against UV-B. Such protective effect was mainly caused by the blockage of UV-B by TiO₂-NPs adsorption. UV-B produced reactive oxygen species (ROS) in the water and in the daphnids, which was not sufficient to cause mortality of daphnids over short periods of radiation. Previous studies focused on the effects of TiO₂-NPs on the toxicity of total UV radiation, and did not attempt to differentiate the potential diverse roles of UV-A and UV-B. Our study indicated that TiO₂-NPs may conversely protect the UV-B toxicity to daphnids. - Highlights: • Interaction between UVB radiation and TiO₂-NPs was studied in Daphnia magna. • Daphnid mortality under UVB decreased in the presence of TiO₂-NPs both in the water and in the body. • Reactive oxygen species produced by UVB was not sufficient to cause mortality of daphnids. • TiO₂-NPs may protect the UVB toxicity to daphnids.