Evaluation of protective role of vitamin E against mono sodium glutamate-induced ovarian damage in female rat model

Objective: To assess the effects of mono sodium glutamate (MSG) on rat's ovaries and to assess if vitamin E had any protective effects. Methodology: This experiment study was conducted at Isra University as well as animal House at Agricultural University, Tando Jam, Sindh. We selected 40 female rats and divided them into four equal groups with 10 rats in each group, after 2 weeks acclimatization period. Group A was on labitum diet, serving as a control group whereas group B was given MSG at 0.08mg/gm dosage till 4 weeks. Group C was given MSG at the same dose but also given vitamin E for 4 weeks, while group D was given MSG for 2 weeks and then Vitamin E for next 2 weeks. Rats were sacrificed with cervical dislocation and ovaries were taken out histological examination under microscope was done after hematoxylin and eosin staining. Results: The ovarian histology was much disturbed in group B, which was given MSG with marked thecal hypertrophy, hemorrhage, follicular destruction along with vacuolization. These changes were observed to a lesser extent in group C animals that were given Vitamin E along with MSG. Group D also showed improvement in these parameters following withdrawal of MSG and administration of vitamin E. Conclusion: Mono sodium glutamate caused ovarian histological damage in rat model and vitamin E had good protective role against it. (author)