Identification of irradiated wheat using paramagnetic electronic resonance and germination test

The objective of this research was to verify the possibility of detection of irradiated wheat by electron spin resonance spectroscopy, because ionizing radiation cause paramagnetic free radicals in materials and the germination test that established in radiobiological effects of inhibition of root-shoot lengths and germination percentages in seeds due radiation. For ESR spectroscopy, grains and shell have been irradiated with doses as follows: 0 kGy ; 1,0 kGy and 10,0 kGy at a dose rate of 6,20 kGy/h. For germination test only grains have been irradiated with 0 kGy; 0,10 kGy; 0,25 kGy; 0,50 kGy; 0,75 kGy; 1,0 kGy and 2,0 kGy at a dose rate of 1,724 kGy/h and stored by 90 days before germination. The ESR signal decay fast with time but shell have free radicals ten times more than grains by mass unit. As we can observe in germination test, occurs a reduction in development of root-shoot and germination percentages with dose increase. The results indicated that the ESR and germination test may be used for detection of irradiated wheat. (author)