Published January 2000 | Version v1
Journal article Metadata-only

A rapid method of reprocessing for electronic microscopy of cut histological in paraffin

Creators

  • 1. Unidad de Microscopia Electronica, Universidad de Costa Rica (Costa Rica)
  • 2. Hospital Nacional de Ninos, San Jose, (Costa Rica)

Description

A simple and rapid method is described for re-processing of light microscopy paraffin sections to observe they under transmission electron microscopy (TEM) and scanning electron microscopy (SEM) The paraffin-embedded tissue is sectioned and deparaffinized in toluene; then exposed to osmium vapor under microwave irradiation using a domestic microwave oven. The tissues were embedded in epoxy resin, polymerized and ultrathin sectioned. The method requires a relatively short time (about 30 minutes for TEM and 15 for SEM), and produces a reasonable quality of the ultrastructure for diagnostic purposes. (Author)

Availability note (English)

Also available from Biblioteca Luis Demetrio Tinoco, Universidad de Costa Rica

Abstract (Spanish)

Se describe un metodo simple y rapido para el re-procesamiento de tejidos incluidos en parafina usados en microscopia de luz para poder ser observados con microscopia electronica de transmision (MET) y de rastreo (MER). Los tejidos incluidos en parafina se seccionaron, desparafinaron en tolueno y se expusieron a vapores de osmio mediante irradiacion con microondas en un horno de microondas domestico. Los tejidos fueron embebidos en resina epoxica, polimerizados y seccionados. El metodo de procesamiento requirio un tiempo relativamente corto (aproximadamente 30 minutos para MET y 15 para MER) y rinde una calidad razonable de la ultraestructura para propositos diagnosticos. (Author)

Additional details

Additional titles

Original title (Spanish)
Un metodo rapido de reprocesamiento para microscopia electronica de corte histologicos en parafina

Publishing Information

Journal Title
Revista Costarricense de Ciencias Medicas
Journal Volume
22
Journal Issue
1-2
Journal Page Range
p. 51-56
ISSN
0253-2948

INIS

Country of Publication
Costa Rica
Country of Input or Organization
Costa Rica
INIS RN
34055306
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
AUTOPSY; BIOPSY; DIAGNOSIS; DIAGNOSTIC TECHNIQUES; HISTOLOGICAL TECHNIQUES; HISTOLOGY; MEDICINE; MYOCARDIUM; OSMIUM; PATHOLOGICAL CHANGES; PATHOLOGY
Descriptors DEC
BODY; CARDIOVASCULAR SYSTEM; DIAGNOSTIC TECHNIQUES; ELEMENTS; HEART; METALS; MUSCLES; ORGANS; PLATINUM METALS; REFRACTORY METALS; TRANSITION ELEMENTS

Optional Information

Notes
Refs., ills., hchavarratcariari.ucr.ac.cr