Multi-functional magnetic molecular imprinting probe for visual detection of IgY antibodies

Egg yolk antibodies (IgY) are an alternative to mammalian antibodies, which have been successfully applied in treatment of disease, as immunochemical reagents and as food additives. A fast, accurate, and easy-to-operate detection method for IgY antibodies is needed. Herein, we developed a rapid and cost-effective colorimetric assay for the ultrasensitive detection of anti-S. aureus IgY antibodies using multi-functional magnetic molecularly imprinted polymers (MMIPs). The prepared MMIPs can recognize, adsorb, and separate the analyte from the matrix efficiently, and oxidize TMB to generate a colorimetric signal within ~ 60 min. As low as 0.013 mg·mL⁻¹ was able to be detected by using this method. The visual detection limit reached 0.02 mg·mL⁻¹. By testing the IgY in milk samples, the feasibility was verified. Graphical abstract: