optimization of insulin radioreceptor assay in human erythrocytes in normal and some disease status

This study is concerned with the evaluation of a new optimized technique for the principle of chloramine-T method used for insulin iodination by 125I-radioisotope with some modifications. The modified procedure can be carried out under normal condition of room temperature, employed longer reaction times and omitted the addition of inorganic reducing salts, maintaining efficient iodination and avoiding denaturations to obtain labels of exceedingly high specific activity on a small quantities of insulin for in vitro usage in the investigation of human erythrocytes 125 I-insulin binding capacity in normal and some disease status