Continuous medium chain carboxylic acids production from excess sludge by granular chain-elongation process

Highlights: • Excess sludge was used to continuously produce MCCAs the first time. • Three branched-MCCAs were obtained and the formation mechanism was revealed. • A small supply of CO₂ facilitated ethanol utilization and the MCCAs yield. • Clostridium sensu stricto and Clostridium IV was the most dominant genera. • Unacidified sludge was unconducive to MCCAs production. Short chain carboxylic acids (SCCAs) production is one of the primary ways to recycle excess sludge (ES). However, the high cost for the SCCAs separation/extraction due to its complete miscibility in water hinders the practical application of SCCAs and the popularization of this recycling way. To overcome this barrier, this study performed an emerging chain elongation (CE) technology to upgrade the SCCAs-rich sludge fermentation broth into the highly hydrophobic medium chain carboxylic acids (MCCAs). In a continuous expanded granule sludge bed (EGSB) reactor, a maximal MCCAs yield of 67.39 % and the corresponding concentration of 9.80 g COD/L (224.97 mM C/L) were achieved. By supplying CO₂ at a loading rate of 2 ${\text{L}}_{{\text{CO}}_2}\text{/(L∙d)}$ to lower the hydrogen partial pressure, the ethanol utilization rate and the resulting MCCAs yield were further improved. In addition, three branched-MCCAs including iso-caproate, iso-heptylate, and iso-caprylate were obtained the first time from waste biomass with the average proportions of 6.17 %, 3.65 %, and 0.8 %, respectively. The branched-MCCAs came from the CE of branched-SCCAs. The granule sludges performing CE were mainly consisted of rod-shaped cells, and dominated by Clostridium sensu stricto and Clostridium IV. This study is expected to lay a foundation for recycling ES to MCCAs.