Published July 30, 2005 | Version v1
Journal article Metadata-only

Purification, crystallization and preliminary crystallographic studies of a calmodulin-OLFp hybrid molecule

Creators

  • 1. Department of Chemistry, National Dong-Hwa University, Hualien 974,Taiwan (China)
  • 2. Institute of Biochemistry, Structural Biology Program, National Yang-Ming University, Shihpai, Taipei 112,Taiwan (China)
  • 3. Department of Medical Research and Education, Veterans General Hospital Taipei, Shihpai, Taipei 112,Taiwan (China)
  • 4. Institute of Molecular Biology, National Chung-Cheng University, Chiayi 520,Taiwan (China)
  • 5. Department of Medical Technology, Tzu Chi University, Hualien 970,Taiwan (China)

Description

The hybrid moelcule of calmodulin and calmodulin-binding domain of olfactory nucleotide-gated ion-channel peptide (CaM-OLFp) was crystallized and preliminary analyzed using X-ray diffaction. A hybrid molecule consisting of calmodulin (CaM) and the CaM-binding domain of olfactory nucleotide-gated ion-channel peptide (CaM-OLFp) was purified and crystallized by the hanging-drop vapour-diffusion method at 298 K. The crystals diffracted to a maximum resolution of 1.85 Å at cryogenic temperature (100 K) using X-rays from a rotating anode (Cu, wavelength 1.54 Å). The crystal belongs to the monoclinic space group C2, with unit-cell parameters a = 64.76, b = 36.23, c = 70.96 Å, α = γ = 90, β = 109.4°. Analysis of the packing density shows that the asymmetric unit contains one CaM-OLFp hybrid molecule with a solvent content of 36.42%

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309105023006; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952352

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952352;
DOI
10.1107/S1744309105023006;
PII
S1744309105023006;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
61
Journal Issue
Pt 8
Journal Page Range
p. 785-787
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46061220
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
ANODES; CRYSTALLIZATION; CRYSTALS; DENSITY; DIFFUSION; HYBRIDIZATION; IONS; MOLECULES; RESOLUTION; SOLVENTS; SPACE GROUPS; STOWING; WAVELENGTHS
Descriptors DEC
CHARGED PARTICLES; ELECTRODES; PHASE TRANSFORMATIONS; PHYSICAL PROPERTIES; SYMMETRY GROUPS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2005
Notes
PMCID: PMC1952352; PMID: 16511158; PUBLISHER-ID: pu5089; OAI: oai:pubmedcentral.nih.gov:1952352