Published June 2015 | Version v1
Journal article

Determination of silver(I) ion based on the aggregation of gold nanoparticles caused by silver-specific DNA, and its effect on the fluorescence of Rhodamine B

  • 1. School of Environmental Science and Engineering, Key Laboratory of Urban Agriculture South, Ministry of Agriculture, School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240 (China)
  • 2. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang, 550025, Huaxi District, Guizhou (China)

Description

We report on an "off-on" type of fluorescent assay for silver(I) ion determination that is based on the use of silver-specific DNA, gold nanoparticles, and Rhodamine B (RhoB). The random coil cytosine-rich silver-specific DNA undergoes structural transformation on capturing Ag(I), and this results in a change in the aggregation status of the gold nanoparticles and simultaneously affects the fluorescence intensity of RhoB that was added to the solution. The fluorescence intensity of RhoB is related to the concentration of Ag(I) in the 2.73 to 1500 ppb range, and the detection limit is 2.73 ppb. The method was applied to the determination of Ag(I) in (spiked) water samples. Conceivably, it also may be applied to fluorescence imaging silver ions and, by using other ion-specific DNAs, to detect other metal ions (author)

Additional details

Identifiers

Publishing Information

Journal Title
Microchimica Acta (Online)
Journal Volume
182
Journal Issue
7-8
Journal Page Range
p. 1411-1419
ISSN
1436-5073