Two-dimensional black phosphorus nanoflakes: A coreactant-free electrochemiluminescence luminophors for selective Pb2+ detection based on resonance energy transfer

Highlights: • a coreactant-free electrochemiluminescence sensing platform was developed. • 2D black phosphorus nanoflakes were exploited as new luminophores. • Ag/AgCl nanocubes were prepared as electrochemiluminescence acceptor. • electrochemiluminescence resonance energy transfer system was presented. As a nondegradable environmental pollutant, lead ion (Pb²⁺) has been proven to be deleterious for environmental and health. Conveniently, quickly and accurately on-site detection of Pb²⁺ is of paramount importance. Herein, an electrochemiluminescence (ECL) assay protocol using two-dimensional black phosphorus (2D BP) nanoflakes as new ECL emitter for highly sensitive and selective trace Pb²⁺ was designed on the basis of Pb²⁺ induced ECL resonance energy transfer (ECL-RET) between 2D BP nanoflakes and Ag/AgCl nanocubes. Anodic green ECL emission of BP nanoflakes without any coreactants was achieved. It is noteworthy that the possible ECL mechanism and the influence of coreactants on the ECL behaviour of BP nanoflakes were further investigated. Benefitting from the well match between the ECL emission spectrum of BP nanoflakes (∼510 nm) and the absorption spectrum of Ag/AgCl nanocubes (200−300 nm and 400−700 nm), effective energy transfer yielded. The introduction of Pb²⁺ would lead to the detachment of Ag/AgCl nanocubes then result in an enhanced ECL emission. Based on this, the proposed method could accurately quantify the Pb²⁺ in the range from 0.5 pM to 5 nM, which exhibited comparative performance to previous work. Furthermore, this study presents the example of employing 2D BP nanoflakes as ECL emitters and constructing a coreactant-free ECL sensing platform, which might open up a promising route for the potential design and implement in clinical analysis.