Application of radiation technology in biomedical materials; fundamentals and applied

1) Immobilization of hemoglobin. Hemoglobin has been immobilized into poly HEMA matrix. To increase, mechanical resistance, at first, CO was coordinated, after immobilization CO was eliminated by photo illumination by visible light from a W lamp and then 02 was introduced. Oxygen coordination ability was not damaged by immobilization. 2) Reduction mechanism of enzyme by the use of pulse radiolysis. Elementary process of reduction mechanism of myoglobin, hemoglobin, HRP and cytochrome oxidase were investigated in the time range of μsec approx. nsec. In the case of cytochrome oxidase, these are 4 metal ions inside of the enzyme. The exact step of reduction of this enzyme was elucidated. (author)