Evaluation of the radioprotective Effect of the co-oral Administration of Vitamin B₁₂ with Vitamin c on some Haematological and Biochemical Alterations in Male Albino Rats

The objective of this study was to evaluate the prophylactic efficacy of co-oral administration of vitamin B₁₂ with vitamin C against radiation induced haematological and biochemical alterations in male albino rats. Male albino rats were divided into six groups (n=8). Group 1: rats were kept as control, Group 2; rats received orally vita-min B₁₂ (2000μgkg^-1). Group 3; rats received Vitamin B₁₂ with Vitamin C (500mgkg^-1). Group 4; rats whole body exposed to 7Gy of gamma rays. Group 5; rats received vitamin B₁₂ for 21 successive days before irradiation. Group 6; rats received Vitamin B₁₂ with Vitamin C for 21 successive days before irradiation. Animals were sacrificed the third day post irradiation. The oral administration of Vitamin B₁₂ with or with-out Vitamin C enhanced the recovery from radiation-induced haemopoietic injury and some biochemical changes demonstrated by a significant increase (p0.05>) of WBCs, RBCs and Platelets count, Hb content, Hct%, serum erythropoietin and iron levels and a significant reduction (p0.05>) of serum homocysteine level (Hcy), creatine kinase (CK-MB) and lactate dehydrogenase (LDH) activities compared to their respective values in irradiated rats. Improvement of oxidative stress in heart and spleen tissues denoted by a significant decrease in lipid peroxidation (MDA) and a significant increase in glutathione (GSH) content was recorded also. The co-oral administration of vitamin B₁₂ with vitamin C has no effect on the prophylactic efficacy of vitamin B₁₂