Serotoninergic receptors in brain tissue: properties and identification of various 3H-ligand binding sites in vitro

In vitro binding studies to serotoninergic receptors were performed using 3H-LSD, 3H-5-HT and 3H-spiperone. An overwiew is given on findings using these three ligands with respect to the following: localization of specific binding sites, in various animal species, the regional distribution in the brain and periphery, the subcellular and cellular distribution. Properties of the binding sites, influence of the composition of the assay medium, binding kinetic properties, receptor regulation in vivo. Identity of the binding sites, differences between site for various 3H-ligands, pharmacological specificity of the membranous binding sites, chemical composition of the macromolecular complex constituting the binding site. Function of the receptor. Binding affinities of 44 compounds were measured in binding assays using 3H-spiperone and 3H-LSD with rat frontal cortex membrane preparations and using 3H-5-HT and 3H-LSD with rat hippocampal membrane preparations