Published 1986 | Version v1
Journal article Metadata-only

Radioimmunoassay of testosterone by using 125I-labelled derivative

Creators

  • 1. Polska Akademia Nauk, Warsaw (Poland)

Description

A method of the determination of testosterone in extracts from human and animal plasma was presented. The antiserum used was directed against testosterone-11α-succinyl-BSA. Testosterone-11α-0-carbonyl-TME was used for labelling with iodine-125 which resulted in two radioactive fractions of slightly differing polarities. Both fractions proved suitable for radioimmunoassay. The amounts of testosterone (in pg/tube), for which the within-assay error did not exceed 12%, ranged from 20 to 600 and from 40 to 1200 for fractions 1 and 2, respectively. The results obtained showed good agreement with those received by using 3H-testosterone (r = 0.986). 7 refs., 3 figs., 1 tab. (author)

Additional details

Additional titles

Original title (Polish)
Radioimmunologiczna metoda oznaczania testosteronu za pomoca pochodnej znakowanej jodem-125

Publishing Information

Journal Title
Endokrynologia Polska
Journal Volume
37
Journal Issue
5
Series
Endokrynol. Pol.
Journal Page Range
219-223
ISSN
0423-104X
CODEN
EDPKA

INIS

Country of Publication
Poland
Country of Input or Organization
Poland
INIS RN
22043476
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ANIMALS; BLOOD PLASMA; CALIBRATION STANDARDS; COMPARATIVE EVALUATIONS; IMMUNE SERUMS; IODINE 125; MAN; RADIOIMMUNOASSAY; TESTOSTERONE; TRITIUM COMPOUNDS
Descriptors DEC
ANDROGENS; ANDROSTANES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EVALUATION; HORMONES; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KETONES; MAMMALS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PRIMATES; RADIOISOTOPES; STEROID HORMONES; STEROIDS; TRACER TECHNIQUES; VERTEBRATES