Published April 1, 1980 | Version v1
Journal article Metadata-only

Specific quantification of mouse and human keratin proteins by radioimmunoassay

Creators

  • 1. National Cancer Inst., Bethesda, MD (USA)
  • 2. Meloy Labs., Springfield, VA (USA)

Description

Mouse epidermal keratin proteins were purified and labelled with 125I by chemical techniques. A radioimmunoassay method was established with a rabbit antibody elicited against the mouse keratin. This assay method was utilized to quantify keratin proteins of mouse and human epidermal extracts, both from intact tissues and cells in culture. As little as 30 ng of mouse keratin proteins (0.5 pmol) was quantifiable. (author)

Additional details

Publishing Information

Journal Title
Biochem. J.
Journal Volume
187
Journal Issue
1
Series
Biochem. J.
Journal Page Range
281-284
ISSN
0006-2936

INIS

Country of Publication
United Kingdom
Country of Input or Organization
United Kingdom
INIS RN
11544036
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ANTIGEN-ANTIBODY REACTIONS; CELL CULTURES; EPIDERMIS; IODINE 125; KERATIN; LABELLED COMPOUNDS; LABELLING; MAN; MICE; RADIOIMMUNOASSAY; SENSITIVITY
Descriptors DEC
ANIMALS; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EPITHELIUM; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PRIMATES; PROTEINS; RADIOISOTOPES; RODENTS; SCLEROPROTEINS; SKIN; TISSUES; TRACER TECHNIQUES; VERTEBRATES