Published October 9, 2004 | Version v1
Journal article Metadata-only

Crystallization and preliminary X-ray characterization of d-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi

Creators

  • 1. Biotechnology Department, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521 (Japan)

Description

d-3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) from P. flagi has been crystallized by the hanging-drop method. A recombinant form of d-3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) from Pseudomonas fragi has been crystallized by the hanging-drop method using PEG 3000 as a precipitating agent. The crystals belong to the orthorhombic group P21212, with unit-cell parameters a = 64.3, b = 99.0, c = 110.2 Å. The crystals are most likely to contain two tetrameric subunits in the asymmetric unit, with a VM value of 3.29 Å3 Da−1. Diffraction data were collected to a 2.0 Å resolution using synchrotron radiation at the BL6A station of the Photon Factory

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309104024741; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952369

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952369;
DOI
10.1107/S1744309104024741;
PII
S1744309104024741;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
61
Journal Issue
Pt 1
Journal Page Range
p. 36-38
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46061237
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CRYSTALLIZATION; CRYSTALS; DIFFRACTION; PHOTONS; RESOLUTION; SYNCHROTRON RADIATION
Descriptors DEC
BOSONS; BREMSSTRAHLUNG; COHERENT SCATTERING; ELECTROMAGNETIC RADIATION; ELEMENTARY PARTICLES; MASSLESS PARTICLES; PHASE TRANSFORMATIONS; RADIATIONS; SCATTERING

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2005
Notes
PMCID: PMC1952369; PMID: 16508084; PUBLISHER-ID: ll5001; OAI: oai:pubmedcentral.nih.gov:1952369