Tritiation and characterization of several suicide substrate enzyme inactivators

Methods are presented to tritiate the enzyme inhibitors pargyline and caracemide. - Highlights: • This paper describes the synthesis of [³H] pargyline and [³H] caracemide. • Several diverse tritiation methods were used to accomplish these labellings and include halogenation–tritium dehalogenation as well as the preparation of [³H] methyl isocyanate. • This paper also describes the characterization of these substances by tritium NMR