Radioimmunoassay for phencyclidine: application to kinetic analysis in the rat
Description
We report the development of a radioimmunoassay for phencyclidine (PCP) that is simple, rapid and sensitive to 0.5 ng/ml. Antibodies were raised in rabbits against the hapten, N-succinyl-3-aminophencyclidine. These antibodies proved to be very specific for PCP and exhibited less than 4% cross reactivity with the drug's two major metabolites. The assay was used for kinetic analysis of PCP in the rat following subcutaneous injection of 5 mg/kg of the drug. Serum and brain tissues were analyzed for PCP and the respective half lives were calculated to be 36 and 29 min for the α phase and 130 and 121 min for the β phase. The accuracy of the method was verified by concomitant assay of a number of kinetic samples by gas chromatography employing a nitrogen-phosphorus detector
Additional details
Publishing Information
- Journal Title
- Life Sci.
- Journal Volume
- 27
- Journal Issue
- 6
- Series
- Life Sci.
- Journal Page Range
- 457-465
- ISSN
- 0024-3205
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 12573632
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE; S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ANESTHETICS; ANTIBODIES; ANTIBODY FORMATION; BIOCHEMICAL REACTION KINETICS; BLOOD PLASMA; BLOOD SERUM; BRAIN; DISTRIBUTION; GAS CHROMATOGRAPHY; METABOLISM; METABOLITES; PHARMACOLOGY; PIPERIDINES; PSYCHOTROPIC DRUGS; RADIOIMMUNOASSAY; RATS; TISSUES; TRITIUM COMPOUNDS
- Descriptors DEC
- AMINES; ANIMALS; AZINES; BIOLOGICAL MATERIALS; BLOOD; BODY; BODY FLUIDS; CENTRAL NERVOUS SYSTEM; CHROMATOGRAPHY; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; MAMMALS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIDINES; REACTION KINETICS; RODENTS; SEPARATION PROCESSES; TRACER TECHNIQUES; VERTEBRATES