Published March 23, 1987
| Version v1
Journal article
19F NMR study of 5-fluorotryptophan-labeled bacteriorhodopsin
Creators
- 1. AN SSSR, Moscow. Inst. Bioorganicheskoj Khimii
Description
19F NMR and CD spectra reveal that bacteriorhodopsin as well as its 5-fluorotryptophan-labeled analog solubilized in a CH3OH-CHCl3 mixture (i) retains a secondary structure of the fully active chromoprotein in the purple membrane and (ii) possesses a folded structure in which modifications at the Lys-216 bound retinal are sensed by sequentially remote tryptophan residues. Individual fragments isolated after limited proteolysis and NaBH4-cleavage of bacteriorhodopsin keep the spatial structure of the intact polypeptide chain in the organic solvent. (Auth.)
Additional details
Publishing Information
- Journal Title
- FEBS (Fed. Eur. Biochem. Soc.) Lett.
- Journal Volume
- 213
- Journal Issue
- 2
- Series
- FEBS (Fed. Eur. Biochem. Soc.) Lett.
- Journal Page Range
- 283-288
- ISSN
- 0014-5793
- CODEN
- FEBLA
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 18076204
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BACTERIA; CELL MEMBRANES; FLUORINE 19; NMR SPECTRA; NUCLEAR MAGNETIC RESONANCE; PROTEIN STRUCTURE; PROTEINS; SPECTROSCOPY
- Descriptors DEC
- CELL CONSTITUENTS; FLUORINE ISOTOPES; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MEMBRANES; MICROORGANISMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RESONANCE; SPECTRA; STABLE ISOTOPES
Optional Information
- Notes
- 16 refs.; 7 figs.