Published October 2004 | Version v1
Journal article Metadata-only

Involvement of cAMP-PKA in regulation of p27Kipl expression induced by ionizing radiation

Creators

  • 1. Beijing Inst. of Radiation Medicine, Beijing (China)

Description

Objective: To study the mechanism of p27Kip1 down-regulation induced by ionizing radiation (IR). Methods: The cDNA of p27Kip1 was cloned into prokaryotic expression vector pGEX4T-2. The recombinant protein GST-p27Kip1 was induced by isopropyl-β-D-thiroglactoside (IPTG) and purified by GST-Sepharose 4B affinity chromatography. Results: GST-p27Kip1 was phosphorylated by PKAc, and this in vitro phosphorylation could be inhibited by PKA specific inhibitor H89. Phosphorylation level of GST-p27Kip1 decreased after IR, when treated with immunoprecipited endogenous PKA. Conclusion: The results suggest that cAMP-PKA plays a critical role in down-regulation of p27Kip1 during IR. (author)

Additional details

Publishing Information

Journal Title
Chinese Journal of Radiological Medicine and Protection
Journal Volume
24
Journal Issue
5
Journal Page Range
p. 393-395
ISSN
0254-5098

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
36107609
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
AMP; CELL CYCLE; COBALT 60; GAMMA RADIATION; IRRADIATION; PHOSPHORYLATION; PHOSPHOTRANSFERASES; PROTEINS; RADIATION DOSES; RADIOINDUCTION
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CHEMICAL REACTIONS; COBALT ISOTOPES; DOSES; ELECTROMAGNETIC RADIATION; ENZYMES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PROTEINS; RADIATIONS; RADIOISOTOPES; TRANSFERASES; YEARS LIVING RADIOISOTOPES

Optional Information

Notes
8 figs., 8 refs.