Published August 15, 1988
| Version v1
Journal article
31P NMR study of daunorubicin-d(CGTACG) complex in solution
- 1. Milan Univ. (Italy). Istituto di Biochmica e Chimica
- 2. Farmitalia Carlo Erba, Milano (Italy). Ricerca e Sviluppo
- 3. CNR, Bologna (Italy). Istituto dei Composti del Carbonio contenenti eteroatomi e loro applicazioni
Description
The interaction of daunorubicin with the self-complementary DNA fragment d(CGTACG) was studied by /sup 31/P NMR spectroscopy. The individual phosphates have been assigned for the nucleotide and the complex and signals from bound and free species in slow exchange at 190C were detected. In solution, the hexanucleotide binds two molecules of daunorubicin, which intercalate in the d(CG) sequence at both ends of the helix. Evidence for local deformations of the backbone at the sites of C/sub 5/pG/sub 6/, C/sub 1/pG/sub 2/and G/sub 2/pT/sub 3/ phosphates is given. The binding constants for the stepwise equilibrium and the rate of dissociation of theh intercalculated duplex were also determined
Additional details
Additional titles
- Subtitle (English)
- Evidence of the intercalation sites
Publishing Information
- Journal Title
- FEBS Letters
- Journal Volume
- 236
- Journal Issue
- 1
- Series
- FEBS Lett.
- Journal Page Range
- 62-70
- ISSN
- 0014-5793
- CODEN
- FEBLA
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 20044963
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ANTIBIOTICS; ANTINEOPLASTIC DRUGS; DNA; DNA ADDUCTS; GLYCOSIDES; NMR SPECTRA; NUCLEAR MAGNETIC RESONANCE; PHOSPHORUS 31; SOLUTIONS
- Descriptors DEC
- ADDUCTS; CARBOHYDRATES; DISPERSIONS; DRUGS; HOMOGENEOUS MIXTURES; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MIXTURES; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; RESONANCE; SPECTRA; STABLE ISOTOPES
Optional Information
- Notes
- 13 refs.; 4 figs.