Assessment and development of time-resolved fluoroimmunoassay for antibody of HCV

To establish an IFMA of anti-HCV, the antigen of HCV was covered on the micro-well plate, and then incubated with human anti-HCV in sample and Eu3+ labeled goat anti-human IgG. The luminescent enhancement solution contains mainly 2-naphthoyl-trifluoro-acetate, and the data was treated with the log-logit function and four-parameter logistic function. Results showed that the intra-and inter-assay CV of the IFMA was 3.64% and 4.39%, respectively, and the recovery rate was 105.58%, the sensitivity was 0.03 NCU/mL. The cross reactivity with HBsAg and HbeAg was 0.23% and 0.04%, respectively. The anti-HCV concentrations in serum of 278 health persons were all less than 1 NCU/mL. Results indicated this new method was sensitive, stable and could be used as an effective non-radioassay tool for the basic or clinical study on HCV