Effects of octacosanol on dopamine uptake of MES23.5 cells induced by MPP+

To assess the effects of octacosanol on [2, 5, 6-3H] dopamine uptake of MES 23.5 cells induced by MPP+, MES23.5 cells were cultured for 24h, and then pre-incubated with octacosanol at different concentrations for 12h. After that, 100 μmol/LMPP+ was added in model and octacosanol groups for 12h. Then the test for [3H] dopamine uptake was performed. After [3H] dopamine uptake test, it was found that [3H] dopamine uptake were significantly decreased in model group (P<0.001) while 10-6 mol/L and 10-7 mol/L octacosanol could partly recover the ability of [3H] dopamine uptake of MES23.5 cells (P<0.05). The results indicate that octacosanol can protect the function of dopamine uptake of MES23.5 cells from MPP+ toxicity. (authors)