Detection of calmodulin binding protein at 170 KDA in BALB, AKR, DON and chicken granulosa cells
Description
Calmodulin (CAM) has been shown to bind to the epidermal growth factor (EGF) receptor (170 kDa) and is phosphorylated in a EGF dependent manner in the A431 human epidermoid carcinoma cells. In the present study, they report 125I-CAM binding to a 170 kDa protein detected in cell membrane vesicles of Balb/3T3, AKR, DON and chicken granulosa cells. Purified plasma membranes from these cells were resolved via electrophoresis (without heat denaturation) and electroblotted onto nictrocellulose paper. Upon hybridizing against 125I-CAM, a distinct autoradiographic band occurred at 170 kDa for all the cells lines under study. The binding of CAM is specific and can be displaced with the addition of excess unlabeled CAM. The result suggest that 125I-CAM may bind to the 170 kDa EGF receptor in BALB, AKR, DON and chicken granulosa cells
Additional details
Publishing Information
- Journal Title
- Fed. Proc., Fed. Am. Soc. Exp. Biol.
- Journal Volume
- 46
- Journal Issue
- 6
- Series
- Fed. Proc., Fed. Am. Soc. Exp. Biol.
- Journal Page Range
- 2000
- ISSN
- 0014-9446
- CODEN
- FEPRA
Conference
- Title
- 78. annual meeting of the American Society of Biological Chemists conference.
- Dates
- 7-11 Jun 1987.
- Place
- Philadelphia, PA (USA).
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18089624
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- ANIMAL CELLS; AUTORADIOGRAPHY; BIOCHEMICAL REACTION KINETICS; CELL MEMBRANES; CHICKENS; ELECTROPHORESIS; IODINE 125; PEPTIDE HORMONES; PROTEINS; RECEPTORS; TRACER TECHNIQUES
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; BIRDS; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; FOWL; HORMONES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MEMBRANES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RADIOISOTOPES; REACTION KINETICS; VERTEBRATES
Optional Information
- Secondary number(s)
- CONF-870644--.