Sex may influence environmental diphenhydramine accumulation in Round Stingrays

Highlights: • Of 18 screened compounds, diphenhydramine was the only quantifiable pharmaceutical • Reference stingrays had no detectable levels of diphenhydramine in liver tissue • Males from the urban site tended to have higher concentrations than females • Sampling location appeared to influence diphenhydramine detection and concentration • Future work should include elasmobranch ecology and physiology as important factors - Abstract: Despite the amount of treated wastewater discharged into the Southern California Bight, few studies have examined pharmaceutical compounds in local biota. The Round Stingray (Urobatis halleri) was selected as a representative elasmobranch species to perform an exploratory study on environmental pharmaceutical exposure. Archived liver samples of males and females from juvenile to adult size classes from several locations (n = 53) were examined for 18 pharmaceutical and illicit drug compounds using isotope-dilution LC-MS/MS. Very few compounds were detected in stingray livers, with diphenhydramine as the only pharmaceutical above quantitation limits. Only stingrays collected from the urban site (mainland California) had detectable levels of diphenhydramine compared to no detections in reference stingrays (offshore island). Sex and sampling location substantially influenced both detection rate and concentrations. Our results suggest that aspects of species' ecology and physiology should be considered for future studies investigating pharmaceutical exposure in elasmobranchs.