Plastisphere enrich antibiotic resistance genes and potential pathogenic bacteria in sewage with pharmaceuticals

Highlights: • The adsorption capacity of TC, AMP and TCS on PVC was higher than that of PE. • PE was corrupted by bacteria, and more conducive to microbial attachment than PVC. • Co-occurrence of TC and MPs led to a higher risk of spreading ARGs and MGEs. • The potential pathogenic bacteria, ARGs and MGEs were enriched on plastisphere. • Legionella, Mycobacterium, Neisseria and Arcobacter might be the hosts for ARGs. Microplastics (MPs) and pharmaceuticals are common emerging pollutants in sewage, and their coexistence may have more negative effects on the environments. This study chose tetracycline (TC), ampicillin (AMP) and triclosan (TCS) to investigate the responses of antibiotic resistance genes (ARGs) and microbial communities on different MPs (polyvinyl chloride (PVC), polyethylene (PE)) biofilms (plastisphere). The adsorption capacity of three pharmaceuticals on PVC and PE decreased in the order of AMP > TC > TCS. PE was more conducive to microbial attachment than PVC. MPs led to the increase of the total copies of ARGs and mobile genetic elements (MGEs) in the sewage. Importantly, multidrug ARGs and MGEs were enriched on plastisphere. Furthermore, the co-occurrence of TC and MPs led to higher risks of spreading ARGs and MGEs. In addition, potential pathogenic bacteria Legionella, Mycobacterium, Neisseria and Arcobacter were more abundant on plastisphere than those in sewage, and these bacteria might be the hosts for ARGs and MGEs. This study showed that plastisphere could be repositories of ARGs and MGEs in sewage and accumulated potential pathogenic bacteria.