Published July 1997 | Version v1
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Plant regeneration from organ culture in white Guinea Yam

  • 1. National Root Crops Research Inst., Umudike, Umuahia, Abia State (Nigeria)
  • 2. Centro Recerche Energia, ENEA, Rome (Italy)

Description

Explants from leaves, leaf segments, petioles and internodal stem of in vitro grown seedlings of white guinea yam, Dioscorea rotundata Poir, cv. 'Obiaoturugo' were cultured on defined media. NAA at concentrations of 0.5-1.0 mg/1 induced shoot regeneration from petiolar and inter-nodal stem pieces, and rooting occurred with little or no callusing from whole leaves or leaf segments. With concentration of 3.0-10.0 m/1 NAA, explants from petioles, inter-nodal stem, whole leaves and leaf segments formed callus which produced roots. These explants developed plantlets when subcultured on MS medium supplemented with 2.0 mg/1 BAP and 0.1 mg/1 NAA. (author). 11 refs, 1 tab

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Improvement of basic food crops in Africa through plant breeding, including the use of induced mutations. Proceedings of a final research co-ordination meeting

Additional details

Publishing Information

Imprint Title
Improvement of basic food crops in Africa through plant breeding, including the use of induced mutations. Proceedings of a final research co-ordination meeting
Imprint Pagination
145 p.
Journal Page Range
p. 99-102.
ISSN
1011-4289
Report number
IAEA-TECDOC--951

Conference

Title
Final research co-ordination meeting on improvement of basic food crops in Africa through plant breeding, including the use of induced mutations.
Dates
30 Oct - 3 Nov 1995.
Place
Naples (Italy).

INIS

Country of Publication
International Atomic Energy Agency (IAEA)
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
28054953
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Conference
Descriptors DEI
BIOLOGICAL REGENERATION; BUDS; GAMMA RADIATION; GUINEA; PLANT GROWTH; TISSUE CULTURES; YAMS
Descriptors DEC
AFRICA; BIOLOGICAL RECOVERY; ELECTROMAGNETIC RADIATION; FOOD; GROWTH; IONIZING RADIATIONS; MAGNOLIOPHYTA; MAGNOLIOPSIDA; PLANTS; RADIATIONS; VEGETABLES

Optional Information

Contract/Grant/Project number
Contract IAEA-R-5432/R4/IG