Metal oxide-chitosan based nanocomposite for cholesterol biosensor

Metal oxide [cerium oxide (NanoCeO2)]-chitosan (CH) nanocomposite film has been fabricated onto indium-tin-oxide (ITO) coated glass plate to immobilize cholesterol oxidase (ChOx) via physiosorption for cholesterol detection. Electrochemical studies reveal that the presence of NanoCeO2 in CH-CeO2 nanocomposite results in increased electroactive surface area for ChOx loading resulting in enhanced electron transport between ChOx and electrode. The ChOx/CH-NanoCeO2/ITO bioelectrode exhibits interesting characteristics such as detection range of 10-400 mg/dL, detection limit of 5 mg/dL, response time of 10 s, low Km value of 3.5 mg/dL and value of regression coefficient of 0.994.