Published November 1977 | Version v1
Journal article Metadata-only

Effects of chloramphenicol and caffeine on postreplication repair in uvrA-umuC- and uvrA-recF- strains of Escherichia coli K-12

Creators

  • 1. Osaka Univ. (Japan). Dept. of Fundamental Radiology

Description

Postreplication repair and its inhibition by chloramphenicol and caffeine, as seen in alkaline sucrose gradients, were compared between a UV nonmutable strain uvrA-umuC- and normally mutable strains uvrA-recF- and uvrA-umu+rec+ of Escherichia coli K-12. The uvrA-umuC- strain performed postreplication repair as efficiently as the parental strain, while the repair in uvrA-recF- strain was dependent on UV dose. Both chloramphenicol and caffeine inhibited postreplication repair to an equal extent of about 25%, and 10%, respectively, in all three uvrA strains of umuC36, recF and umu+rec+. These observations suggest that postreplication repair is largely not responsible for UV mutagenesis. (orig.)

Abstract (German)

Die Postreplikationsreparatur und ihre mit Hilfe des alkalischen Sucrose-Gradienten nachzuweisende Hemmung durch Chloramphenicol und Koffein wurden an einem nicht UV-mutierbaren Stamm uvrA-umuC- und normal mutierenden Staemmen uvrA-recF- und uvrA-umu+rec+ von E. coli K-12 untersucht. Bei dem Stamm uvrA-umuC- verlief die Postreplikationsreparatur ebenso vollstaendig wie beim Elternstamm; beim Stamm uvrA-recF- dagegen war die Reparatur von der UV-Doris abhaengig. Chloramphenicol und Koffein hemmten die Postreplikationsreparatur gleichmaessig um 25% bzw. 10% bei allen drei uvrA--Staemmen von umuC36, recF und umu+rec+. Diese Beobachtungen lassen vermuten, dass die Postreplikationsreparatur fuer die UV-Mutagenese weitgehend nicht die Ursache ist. (orig.)

Additional details

Identifiers

DOI
10.1007/bf00283483;

Publishing Information

Journal Title
Molecular and General Genetics MGG
Journal Volume
156
Journal Issue
2
Series
Mol. Gen. Genet.
Journal Page Range
115-120
ISSN
0026-8925

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
9376078
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL REPAIR; CAFFEINE; CHLORAMPHENICOL; ESCHERICHIA COLI; INHIBITION; MUTAGENESIS; ULTRAVIOLET RADIATION
Descriptors DEC
ANTIBIOTICS; BACTERIA; BIOLOGICAL RECOVERY; DRUGS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; MICROORGANISMS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PURINES; RADIATIONS; XANTHINES

Optional Information

Notes
3 figs.; 1 tab.; 25 refs.; Updated automatically by Metadata and Full-Text Enrichment Agent