Published 1973 | Version v1
Journal article Metadata-only

A simplified biological synthesis of labelled cholesteryl esters

Creators

  • 1. Pennsylvania Univ., Philadelphia (USA)

Description

Abstract A simplified procedure is described for the synthesis and purification of labelled cholesteryl esters. The esters are synthesized enzymatically from (4‐ 14 C) cholesterol and free fatty acids. The separation of the lipids from aqueous medium is simplified by lyophilizing the reaction mixture at equilibrium. The lipid components of the freeze‐dried powder are separated by silicic acid chromatography. The radiochemical purity of the product was shown to be greater than 98%.

Additional details

Identifiers

DOI
10.1002/jlcr.2590090221;

Publishing Information

Journal Title
Journal of Labelled Compounds
Journal Volume
9
Journal Issue
2
Series
J. Labelled Compd.
Journal Page Range
365-371
ISSN
0022-2135

INIS

Country of Publication
Belgium
Country of Input or Organization
Belgium
INIS RN
5100797
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
Descriptors DEI
CARBON 14 COMPOUNDS; CHEMICAL PREPARATION; CHEMICAL REACTION YIELD; ESTERASES; ESTERS; LIPIDS
Descriptors DEC
ENZYMES; HYDROLASES; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; RADIOACTIVE MATERIALS; SYNTHESIS

Optional Information

Notes
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