The effect of Ginkgo biloba extract on parkinsonisminduced biochemical changes in brain of irradiated rats
- 1. Radiation Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo (Egypt)
Description
Parkinson's disease (PD) is the second most common neuro degenerative disorder after Alzheimer's disease. In the present study, neuro modulatory effects of standardized ginkgo biloba extract (EGb 761) and low dose whole-body γ-irradiation in a reserpine model of rat Parkinsonism were investigated. Male Wistar rats were pretreated orally with EGb 761 (100 mg/kg BW/day for 3 weeks) or low dose whole-body γ-irradiation (0.25 Gy once a week for 6 weeks) and their combination (EGb 761 was received during the last three weeks of the irradiation period) and then subjected to intraperitoneal injection of reserpine (5 mg/kg BW dissolved in 1% acetic acid) 24h after last dose of EGb761or radiation. All rats were sacrificed 24h after reserpine injection. Depletion of striatal dopamine (DA) level, increased oxidative stress indicated via depletion of glutathione (GSH), increased malondialdehyde (MDA) and iron levels; decrease of dopamine metabolites metabolizing enzymes; indicated by decrease of glutathione-S transferase (GST) and NADPH-quinone oxidoreductase (NQO) activities; mitochondrial dysfunction; indicated by decline of complex I activity and adenosine triphosphate (ATP) level and increased apoptosis; indicated by the decrease of mitochondrial B cell lymphoma-2 protein (Bcl-2) level and as shown by transmission electron microscope (TEM) were observed in brain of reserpine-induced PD model group, along with behavioral study indicated by increased catalepsy score. Moreover, the level of GSH was correlated with the levels of both DA (r = 0.78) and MDA (r = -0.93). The level of Bcl-2 was correlated with the complex I activity (r = 0.94) and ATP level (r = 0.98). Results revealed that either EGb 761 or irradiation and their combination ameliorated most of the biochemical and behavioral changes induced by reserpine possibly via replenishment of normal glutathione levels. This study revealed that EGb 761, which is a widely used herbal medicine and low dose of whole-body γ-irradiation have protective potential in combating Parkinsonism and can be used in preventing this devastating neurologic disorder.
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Additional details
Publishing Information
- Imprint Pagination
- 152 p.
- Report number
- INIS-EG--392
INIS
- Country of Publication
- Egypt
- Country of Input or Organization
- Egypt
- INIS RN
- 45099945
- Subject category
- S60: APPLIED LIFE SCIENCES; S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Resource subtype / Literary indicator
- Thesis
- Quality check status
- Yes
- Descriptors DEI
- ATP; BIOCHEMISTRY; BRAIN; GAMMA RADIATION; GLUTATHIONE; NERVE CELLS; PLANTS; RATS; RESERPINE; TRANSMISSION ELECTRON MICROSCOPY;
- Descriptors DEC
- ALKALOIDS; ANIMAL CELLS; ANIMALS; ANTIHYPERTENSIVE AGENTS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZAARENES; AZOLES; BODY; CARDIOVASCULAR AGENTS; CENTRAL NERVOUS SYSTEM; CENTRAL NERVOUS SYSTEM AGENTS; CENTRAL NERVOUS SYSTEM DEPRESSANTS; CHEMISTRY; DRUGS; ELECTROMAGNETIC RADIATION; ELECTRON MICROSCOPY; HETEROCYCLIC COMPOUNDS; HYPNOTICS AND SEDATIVES; INDOLES; IONIZING RADIATIONS; MAMMALS; MICROSCOPY; NERVOUS SYSTEM; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PEPTIDES; POLYPEPTIDES; PROTEINS; PSYCHOTROPIC DRUGS; PYRROLES; RADIATIONS; RADIOPROTECTIVE SUBSTANCES; RESPONSE MODIFYING FACTORS; RODENTS; SOMATIC CELLS; SYMPATHOLYTICS; TRANQUILIZERS; VERTEBRATES;
Optional Information
- Notes
- 10 tabs., 27 figs., 410 refs.