Published May 2001 | Version v1
Journal article Metadata-only

In vitro culture of soybean immature cotyledons and genetic transformation via biolistic system

Creators

  • 1. Institute for Application of Atomic Energy, Zhejiang Academy of Agricultural Sciences, Hangzhou (China)

Description

40 soybean (Glycine max (L.) Merr.) cultivars were collected to study in vitro culture of immature cotyledons. Regenerated plants of 3 cultivars were obtained and one cultivar was used to study the effect of irradiation treatment on stable genetic transformation rate. O, 5, 10, 20 Gy were applied before or after bombardment with Bio-Rad system. It was found that 5 Gy was the effective dose to raise the ratio of cotyledons with KmR calli. GUS enzyme activity showed that foreign genes had been integrated into genome of soybean callus. (authors)

Additional details

Publishing Information

Journal Title
Journal of Nuclear Agricultural Sciences
Journal Volume
15
Journal Issue
5
Journal Page Range
p. 282-285
ISSN
1000-8551

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
48045497
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
GENES; GENETICS; IN VITRO; IRRADIATION; LEAVES; RADIATION DOSE UNITS; SOYBEANS
Descriptors DEC
BIOLOGY; FOOD; PLANTS; SEEDS; UNITS; VEGETABLES

Optional Information

Notes
3 figs., 2 tabs., 11 refs.