Effects of elevated CO2 and endophytic bacterium on photosynthetic characteristics and cadmium accumulation in Sedum alfredii

Highlights: • Elevated CO₂ improved the colonization of endophytic bacterium SaMR12. • eCO₂ × SaMR12 interaction had positive effects on S. alfredii growth and Cd accumulation. • eCO₂ × SaMR12 interaction increased photosynthetic carbon uptake. • eCO₂ × SaMR12 interaction promoted electron transport and PS II activity. • eCO₂ × SaMR12 interaction upregulated the expressions of heavy metal transporter. Elevated CO₂ and use of endophytic microorganisms have been considered as efficient and novel ways to improve phytoextraction efficiency. However, the interactive effects of elevated CO₂ and endophytes on hyperaccumulator is poorly understood. In this study, a hydroponics experiment was conducted to investigate the combined effect of elevated CO₂ (eCO₂) and inoculation with endophyte SaMR12 (ES) on the photosynthetic characteristics and cadmium (Cd) accumulation in hyperaccumulator Sedum alfredii. The results showed that eCO₂ × ES interaction promoted the growth of S. alfredii, shoot and root biomass net increment were increased by 264.7 and 392.3%, respectively, as compared with plants grown in ambient CO₂ (aCO₂). The interaction of eCO₂ and ES significantly (P < 0.05) increased chlorophyll content (53.2%), Pn (111.6%), Pn_max (59.8%), AQY (65.1%), and Lsp (28.8%), but reduced Gs, Tr, Rd, and Lcp. Increased photosynthetic efficiency was associated with higher activities of rubisco, Ca²⁺-ATPase, and Mg²⁺-ATPase, and linked with over-expression of two photosystem related genes (SaPsbS and SaLhcb2). PS II activities were significantly (P < 0.05) enhanced with Fv/Fm and Φ(II) increased by 12.3 and 13.0%, respectively, compared with plants grown in aCO₂. In addition, the net uptake of Cd in the shoot and root tissue of S. alfredii grown in eCO₂ × ES treatment was increased by 260.7 and 434.9%, respectively, due to increased expression of SaHMA2 and SaCAX2 Cd transporter genes. Our results suggest that eCO₂ × ES can promote the growth of S. alfredii due to increased photosynthetic efficiency, and improve Cd accumulation and showed considerable potential of improving the phytoextraction ability of Cd by S. alfredii.