Modification in nucleocytoplasmic transport of RNA in vitro after whole-body X-irradiation
Creators
- 1. Centre d'Etude de l'Energie Nucleaire, Mol (Belgium). Dept. de Radiobiologie
- 2. Liege Univ. (Belgium). Lab. de Biochimie Appliquee
Description
Rats were sacrificed at different times after injection of 14C-orotate and liver nuclei were isolated and incubated in a cellfree system made of soluble cytoplasmic fraction from non-irradiated rat livers. The release of ribonucleoproteins from labelled nuclei to the incubation medium was measured on nuclei from normal and total-body irradiated rats (500 to 2.000 R). When using short labelling times in vivo (30 min), which would label predominatly messenger-type RNP, the nuclear transport activity was depressed at early times (3 h) after whole-body irradiation, but increased at later times (24 h). When labelling time in vivo is increased to 2 h, no significant difference was observed between control and irradiated groups. Transport of the stable ribosomal-type RNP, observed 24 h after both labelling and irradiation, was depressed. These results are discussed in view of previous personal studies in radiation effect on liver RNA synthesis and polyribosomes formation in vivo. (orig.)
Abstract (German)
Ratten wurden zu verschiedenen Zeiten nach Injektion von 14C-Orotat getoetet, die Leberzellkerne wurden isoliert und in einem zellfreien System aus loeslichem Zytoplasma von unbestrahlter Leber inkubiert. Die Abgabe von Ribonukleoprotein durch die markierten Zellkerne an das Inkubationsmedium wurde fuer Kerne von normalen und ganzkoerperbestrahlten Ratten bestimmt. Fuer kurze in vivo Markierungsperioden (30 min), wenn hauptsaechlich Boten-RNS markiert ist, zeigte sich bald (3 Stunden) nach Ganzkoerperbestrahlung eine Abnahme der Transportaktivitaet der Kerne und spaeter eine Erhoehung. Bei einer in vivo Markierungszeit von 2 Stunden liess sich kein signifikanter Unterschied zwischen Kontroll- und bestrahlten Tieren feststellen. Der Transport stabilen Nukleoproteins (24 Stunden nach Markierung und Ganzkoerperbestrahlung) war vermindert. Die Ergebnisse werden in Hinblick auf die frueher gefundenen Wirkungen von Bestrahlung auf die Leber-RNS und Polysomenbildung diskutiert. (orig.)Additional details
Publishing Information
- Journal Title
- Strahlentherapie
- Journal Volume
- 153
- Journal Issue
- 11
- Series
- Strahlentherapie.
- Journal Page Range
- 769-773
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 9367481
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ANIMAL CELLS; BIOCHEMICAL REACTION KINETICS; BIOLOGICAL RADIATION EFFECTS; CARBON 14 COMPOUNDS; CELL NUCLEI; CYTOPLASM; IN VITRO; IN VIVO; LIVER; OROTIC ACID; RATS; RIBOSOMES; RNA; TRACER TECHNIQUES; TRANSFER RNA; WHOLE-BODY IRRADIATION; X RADIATION
- Descriptors DEC
- ANIMALS; AZINES; BIOLOGICAL EFFECTS; BODY; CARBON COMPOUNDS; CARBOXYLIC ACIDS; CELL CONSTITUENTS; DIGESTIVE SYSTEM; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; GLANDS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; IONIZING RADIATIONS; IRRADIATION; ISOTOPE APPLICATIONS; KINETICS; MAMMALS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANOIDS; ORGANS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; REACTION KINETICS; RODENTS; URACILS; VERTEBRATES
Optional Information
- Notes
- 1 fig.; 1 tab.; 26 refs.