Preparation of radioiodine-131 labeled apigenin anti cancer compound for bioactivity study

Apigenin is a flavonoid derivative that has potential as a candidate of anti-cancer, anti-oxidant, and anti-inflammatory with low intrinsic toxicity. To determine the bioactivity of apigenin can be carried out by the radioiodination method and continue with a preclinical study using test animals. This research was conducted to prepared a ¹³¹I-Apigenin using the chloramine-T method and optimizing the parameters of radiolabeling. In this study, ¹³¹I-Apigenin was prepared with the formulation of 1 mg apigenin, 250 μg chloramine-T, and 100 μg sodium metabisulfite in pH 7 with an addition 10 μL (10 μCi) of radioiodine-131. The electrophilic substitution reaction was quite rapid within 20 minutes at room temperature(20-22 °C). The radiochemical purity was confirmed by thin-layer chromatography using Whatman 1 as static phase and ammonium acetate 0,02 M, pH 6 as mobile phase, the radiochemical purity was obtained 96,53 ± 1,87 %. The result would make ¹³¹I-Apigenin continue for physicochemical and preclinical study to discover its bioactivity and effectiveness as an anti-cancer natural compound. (author)