Synthesis, radiometal labeling and in vitro evaluation of a targeted PPIX derivative
- 1. Department of Chemistry, University of Western Ontario, 1151 Richmond Street, London, Ontario, N6A 5B7 (Canada)
- 2. Department of Medical Biophysics, University of Western Ontario, 1151 Richmond Street, London, Ontario, N6A 5B7 (Canada)
- 3. London Regional Cancer Program, 790 Commissioners Road East, London, Ontario, N6A 4L6 (Canada)
- 4. Department of Surgery, University of Western Ontario, 1151 Richmond Street, London, Ontario, N6A 5B7 (Canada)
- 5. Department of Oncology, University of Western Ontario, 1151 Richmond Street, London, Ontario, N6A 5B7 (Canada)
- 6. Department of Medical Imaging, University of Western Ontario, 1151 Richmond Street, London, Ontario, N6A 5B7 (Canada)
Description
In pursuit of a single molecule with potential applications for both in vitro fluorescence microscopy and in vivo PET imaging, novel targeted 69/71Ga/68Ga-protoporphyrin IX (PPIX) probes were developed. Optical analysis, fluorescence microscopy and radiolabeling with gallium-68 were performed to confirm potential applications. The use of a targeted-PPIX ring, in conjunction with 69/71Ga or 68Ga, eliminates the need for the attachment of multiple imaging tags, allowing for either in vitro fluorescent-based evaluation or in vivo nuclear-based imaging. - Highlights: ► Synthesized and characterized αvβ3 targeted 69/71Ga/68Ga-PPIX analogues. ► Gallium chelation does not quench fluorescence. ► In vitro fluorescence microscopy indicates specific uptake of tracer. ► Gallium-68 labeling of PPIX is optimal in a microwave reactor.
Availability note (English)
Available from http://dx.doi.org/10.1016/j.apradiso.2011.11.054Additional details
Identifiers
- DOI
- 10.1016/j.apradiso.2011.11.054;
- PII
- S0969-8043(11)00608-7;
Publishing Information
- Journal Title
- Applied Radiation and Isotopes
- Journal Volume
- 70
- Journal Issue
- 3
- Journal Page Range
- p. 505-511
- ISSN
- 0969-8043
- CODEN
- ARISEF
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 43072941
- Subject category
- S07: ISOTOPES AND RADIATION SOURCES;
- Descriptors DEI
- FLUORESCENCE; GALLIUM 68; GALLIUM 69; GALLIUM 71; IN VIVO; LABELLING; MICROSCOPY; PORPHYRINS; RADIOPHARMACEUTICALS; SYNTHESIS
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EMISSION; GALLIUM ISOTOPES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; ISOTOPES; LABELLED COMPOUNDS; LUMINESCENCE; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOTON EMISSION; RADIOACTIVE MATERIALS; RADIOISOTOPES; STABLE ISOTOPES
Optional Information
- Copyright
- Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.