Interrelation of the cell cycle dynamics and chromosome aberration frequency in the culture of human irradiated lymphocytes

Using lymphocytes of human blood irradiated by Co60 gamma rays in the doses of 150 and 300 rad (dose rate 600 rad/min) the interrelation of cell divisions and chromosome aberration frequency during different periods of culture fiscation of man's lymphacytes in the norm and upon gamma irradiation in vitro has been studied. The cells have fixed in 48, 56, 68 and 80 h after cultivation start. It is shown on the whole that quantitative regularities of radiation-induced chromosome aberrations cannot be studied without cell identification of the 1-st and subsequent divisions. It has been found that the asynchronization in controlled and irradiated cultures of man's lymphocytes grows with increase of cultivation periods. Already at the 48sub(th) hour of cultivation the culture represents a mixture of cells, of two first divisions, whereas at the 80sup(-th) - five divisions. Gamma irradiation of nonstimulated lymphocytes causes mitotic delay the duration of which is directly proportional to the radiation effect dose and constitutes about 3h per 100 rad. The frequency of radiation-induced chromosome aberrations and cells with aberrations does not differ for certain in cells passing the 1-st, 2-nd and 3-rd division in 48, 56, 68 and 80 h after cultivaton start