Cellular response to nano-structured Zr and ZrO2 alloyed layers on Ti-6Al-4V

Highlights: • Nano-structured Zr(Zr@TC4)and micro/nano-structured ZrO₂ (ZrO₂@TC4)alloyed layers were fabricated on Ti6Vl4V. • Zr@TC4 decreases the hydrophilicity whereas ZrO₂@TC4 is more hydrophilic than TC4. • Compare to TC4, more proteins adsorb on ZrO₂@TC4 and Zr@TC4. • Zr@TC4 promotes the cellular activity including spreading of osteoblasts, while ZrO₂@TC4 restricts cell adhesion and spreading of osteoblasts and endothelial cells. - Abstract: The surface topography of biomaterials is known to influence cellular response such as adhesion, spreading and differentiation. In this work, the behavior of osteoblasts and endothelial cells on nano-structured Zr and micro/nano-structured ZrO₂ alloyed layers of Ti-6Al-4V (TC4) was investigated. Zr alloyed layer (Zr@TC4) decreases the hydrophilicity whereas ZrO₂ alloyed layers (ZrO₂@TC4) is more hydrophilic than TC4 and more proteins adsorb on ZrO₂@TC4 followed by Zr@TC4. The cells proliferate steadily on the smooth TC4 and nano-structured Zr@TC4 surfaces and the osteoblast activity is more pronounced on Zr@TC4 than TC4. The micro/nano-structured surface on ZrO₂@TC4 restricts cellular adhesion and spreading independent of the surface wettability and protein adsorption. The findings provide insights into the design of micro/nano-structured biomaterials and interfaces with controlled tissue response.