Silver nanoparticles alter learning and memory formation in an aquatic organism, Lymnaea stagnalis

We tested the effect of silver nanoparticles (AgNPs) on the ability of the pond snail, Lymnaea stagnalis, to learn and form long-term memory (LTM) following operant conditioning of aerial respiration. We hypothesized that the AgNPs would act as a stressor and prevent learning and LTM formation. We tested snails exposed for either 72 h or only during training and testing for memory (i.e. 0.5 h) and found no difference between those treatments. We found that at a low concentration of AgNPs (5 μg/L) neither learning and nor memory formation were altered. When we increased the concentration of AgNPs (10 μg/L) we found that memory formation was enhanced. Finally, at a higher concentration (50 μg/L) memory formation was blocked. To determine if the disassociation of Ag⁺ from the AgNPs caused the effects on memory we performed similar experiments with AgNO₃ and found similar concentration-dependent results. Finally, we found that snails perceive the AgNPs differently from Ag+ as there was context specific memory. That is, snails trained in AgNPs did not show memory when tested in Ag⁺ and vice-versa. We believe that changes in memory formation may be a more sensitive determination of AgNPs on aquatic organisms than the determination of a LC₅₀. - Highlights: • A higher concentration (10 μg/L) enhances memory formation. • The highest concentration (50 μg/L) blocks memory formation. • Chronic and acute exposures were no different. • AgNO₃ concentrations (1, 5 and 10 μg/L respectively) have similar effects. • Memory recall is context specific, thus snails trained in AgNPs do not exhibit memory when tested in AgNO₃ and vice-versa.