Published December 2008
| Version v1
Journal article
High frequency plant regeneration from leaf explants derived callus of evening primrose (oenothera biennis)
Creators
- 1. Pakistan Councile of Scientific and Industrial Research Labs., Peshawar (Pakistan). Dept. of Medicinal Botanic
- 2. Pakistan Councile of Scientific and Industrial Research Labs., Lahore (Pakistan). Dept. of Food and Biotechnology
Description
The seeds of Evening primrose were aseptically grown and leaf explants were used for establishment of callus culture. The Excellent growth in callus biomass was achieved on MS medium supplemented with 2, 4, -D and TDZ. For optimal growth of bud and shoot regeneration, fortification of IAA along with TDZ, or BAP was found to be essential. Rooting (70%) could be inducted on hormone free MS-medium. This percentage improved to 98 when NAA was added to the medium. The plantlets thus obtained were transferred to the field successfully after passing through the process of hardening. (author)
Additional details
Publishing Information
- Journal Title
- Pakistan Journal of Biochemistry and Molecular Biology
- Journal Volume
- 41
- Journal Issue
- 4
- Journal Page Range
- p. 158-161
- ISSN
- 1681-4525
INIS
- Country of Publication
- Pakistan
- Country of Input or Organization
- Pakistan
- INIS RN
- 40047292
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BIOLOGICAL REGENERATION; GERMINATION; LEAVES; MEDICINAL PLANTS; SEEDS; TISSUE CULTURES
- Descriptors DEC
- BIOLOGICAL RECOVERY; PLANTS