Effect of gamma irradiation on erythrocyte membrane in rats by fluorescent probes in vitro and in vivo
Creators
- 1. Academy of Military Medical Sciences (PLA), Beijing, BJ (China). Inst. of Radiation Medicine
Description
Fluorescent dyes, 1-anilino-naphthalene sulfonate (ANS) and 1,6-diphenyl-1,3,5-hexatriene (DPH), were used as probes to examine the effect of γ-irradiation on the rat erythrocyte membrane. After irradiation of rat erythrocyte membrane in vitro, the fluorescence intensity of membrane-associated ANS decreased with the increase of irradiation dose (10-40 Gy). Similar results were also observed in the experiment of irradiation in vivo as showed by the decrease of fluorescence intensity of DPH probe in the erythrocyte membrane as well as the increase of its polarization on the 1 st, 3 rd, 5 th day following whole body irradiation with 8.5 Gy. Those results reflected the decrease of membrane fluidity which was suggested to be resulted from lipid peroxidation of polyunsaturated fatty acid in membrane induced by γ-irradiation in vitro and in vivo
Additional details
Publishing Information
- Journal Title
- Journal of Radiation Research and Radiation Processing
- Journal Volume
- 8
- Journal Issue
- 4
- Series
- J. Radiat. Res. Radiat. Process.
- Journal Page Range
- 199-202
- ISSN
- 1000-3436
- CODEN
- FYYXE
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 22083965
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
- Descriptors DEI
- ANILINE; BIOLOGICAL RADIATION EFFECTS; CELL MEMBRANES; DOSE-RESPONSE RELATIONSHIPS; ERYTHROCYTES; FLUID FLOW; FLUORESCENCE SPECTROSCOPY; GAMMA RADIATION; IN VITRO; IN VIVO; POLYENES; RATS; SULFONIC ACIDS; WHOLE-BODY IRRADIATION
- Descriptors DEC
- AMINES; ANIMALS; AROMATICS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL CONSTITUENTS; ELECTROMAGNETIC RADIATION; EMISSION SPECTROSCOPY; EXTERNAL IRRADIATION; HYDROCARBONS; IONIZING RADIATIONS; IRRADIATION; MAMMALS; MATERIALS; MEMBRANES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RODENTS; SPECTROSCOPY; VERTEBRATES