The assay of thyrotropin receptor antibodies with human TSH/LH-CG chimeric receptor expressed on chinese hamster ovary cells
Creators
- 1. Korea Cancer Center Hospital, Seoul (Korea, Republic of)
Description
TSH/LH-CG chimera cDNA is transfected to CHO-K1 cell to obtain the chimeric receptor expressed on the cell surface. The optimal conditions for TSAb and TSBAb measurements are determined using chimeric receptors and under these conditions activity of TSAb and TSBAb in the sera of the Graves' patients. The results obtained are compared to those of TSAb assays using FRTL5 cells CHO-TSHR cells which have wild type human TSH receptor. The transfection procedure of chimeric receptor gene to CHO-K1 cells are on going. The optimal conditions for TSAb and TSBAb measurement using chimeric receptor will be determined after success of transfection procedure. If this study is successfully completed, not only the heterogeneity of Graves. IgG but also pathogenesis of Graves' disease will be elucidated. (author). 25 refs
Availability note (English)
Available from INIS in electronic form and/or on microfiche .Files
29000806.pdf
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Additional details
Publishing Information
- Imprint Pagination
- 46 p.
- Report number
- KAERI/RR--1654/96
INIS
- Country of Publication
- Korea, Republic of
- Country of Input or Organization
- Korea, Republic of
- INIS RN
- 29000806
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ANTIBODIES; CHIMERAS; CHO CELLS; PATIENTS; RECEPTORS; THYROID HORMONES; TSH
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; HORMONES; MAMMALS; MAN; MOSAICISM; PEPTIDE HORMONES; PITUITARY HORMONES; PRIMATES; SOMATIC CELLS; VERTEBRATES