Scintillation proximity radioimmunoassay utilizing 125I-labeled ligands
- 1. Roche Institute of Molecular Biology, Nutley, NJ
Description
A unique type of radioimmunoassay is described that does not require centrifugation or separation. Microbeads containing a fluorophor are covalently linked to antibody. When an 125I-labeled antigen is added it binds to the beads and, by its proximity, the emitted short-range electrons of the 125I excite the fluorophor in the beads. The light emitted can be measured in a standard scintillation counter. Addition of unlabeled antigen from tissue extracts displaces the labeled ligand and diminishes the fluorescent signal. Application of scintillation proximity immunoassay to tissue enkephalins, serum thyroxin, and urinary morphine is described. Applications of the principle to study the kinetics of interaction between receptors and ligands are discussed
Additional details
Publishing Information
- Journal Title
- Proc. Natl. Acad. Sci. U.S.A
- Journal Issue
- no.24
- Series
- Proc. Natl. Acad. Sci. U.S.A.
- ISSN
- 0027-8424
- CODEN
- PNASA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 17068696
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ENKEPHALINS; IODINE 125; LABELLED COMPOUNDS; MORPHINE; RADIOIMMUNOASSAY; SCINTILLATION COUNTING; THYROXINE
- Descriptors DEC
- ALKALOIDS; AMINO ACIDS; ANALGESICS; AUTONOMIC NERVOUS SYSTEM AGENT; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM AGENTS; CENTRAL NERVOUS SYSTEM DEPRESS; COUNTING TECHNIQUES; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENDORPHINS; HORMONES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NARCOTICS; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC IODINE COMPOUNDS; PEPTIDE HORMONES; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOISOTOPES; THYROID HORMONES; TRACER TECHNIQUES