Published February 1, 1985
| Version v1
Journal article
Basolateral membrane of rat enterocyte: its purification from brush border contamination
Description
Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaCl2 to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low L-glucose permeability across basolateral membrane vesicles together with ATP-dependent sodium uptake was observed
Additional details
Publishing Information
- Journal Title
- Anal. Biochem.
- Journal Issue
- no.2
- Series
- Anal. Biochem.
- ISSN
- 0003-2697
- CODEN
- ANBCA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 17016530
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- CALCIUM CHLORIDES; CELL MEMBRANES; CENTRIFUGATION; CYTOLOGICAL TECHNIQUES; GLUCOSE; INTESTINES; PERMEABILITY; RATS; SODIUM COMPOUNDS; SODIUM 22; TRACER TECHNIQUES
- Descriptors DEC
- ALDEHYDES; ALKALI METAL COMPOUNDS; ALKALINE EARTH METAL COMPOUNDS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; CALCIUM COMPOUNDS; CALCIUM HALIDES; CARBOHYDRATES; CELL CONSTITUENTS; CHLORIDES; CHLORINE COMPOUNDS; DIGESTIVE SYSTEM; GASTROINTESTINAL TRACT; HALIDES; HALOGEN COMPOUNDS; HEXOSES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MEMBRANES; MONOSACCHARIDES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; RADIOISOTOPES; RODENTS; SACCHARIDES; SEPARATION PROCESSES; SODIUM ISOTOPES; VERTEBRATES; YEARS LIVING RADIOISOTOPES