Peculiarities of γ-radiation action on collagen amino acid composition under modifying effect of gas atmosphere

The comparative changes in the amino acid composition of calf skin collagen after γ-irradiation (doses from 100 to 1.000 Gy) in aqueous solutions under different gas atmospheres (O2, N2O, H2, vacuum) were investigated. The radiochemical yields of collagen amino acid residues destruction were determined. Under O2(OH, O2-) most of amino acids are destroyed with higher yields than under N2O. Leucine, valine, isoleucine, phenylalanine, arginine were the exception because of their high reaction rate constants with OH and hydroxylation reactions. Under H2 (e-aq, H) and in vacuum (e-aq, OH) the mechanism of collagen radiolysis changed due to its aggregation; the destruction of those amino acids which have high reaction rate constants with water radiolysis products was mainly observed (phenylalanine, tyrosine, histidine)