Published August 2010 | Version v1
Journal article

Establishment of an Enzyme Linked Immunosorbent Assay for Albumin

  • 1. Department of Isotope, China Institute of Atomic Energy, Beijing (China)

Description

A sensitive and specific ELISA for Albumin was established by using Albumin antibody coated on the microtiter plate, Albumin labeled horseradish peroxidase(HRP). The sensitivity of the assay was 0.28 mg/L. The intra-and inter-assay CVs were 2.63%-5.28% and 2.40%-4.26% respectively. The analytical recovery was 95.0%-106.4%, the coefficient correlation was 0.9974. This method for measuring Alb was rapid, sensitive and convenient. It is suitable for clinical detection and research application. (authors)

Additional details

Publishing Information

Journal Title
Journal of Isotopes
Journal Volume
23
Journal Issue
3
Journal Page Range
p. 182-185
ISSN
1000-7512

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
44076082
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ALBUMINS; ANTIBODIES; CORRELATIONS; DETECTION; ENZYME IMMUNOASSAY; PEROXIDASES; SENSITIVITY
Descriptors DEC
BIOASSAY; ENZYMES; IMMUNOASSAY; ORGANIC COMPOUNDS; OXIDOREDUCTASES; PROTEINS

Optional Information

Notes
1 figs., 5 tabs., 6 refs.