Published September 2007 | Version v1
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Effects of ionizing radiation on purinergic signaling modulation in rat brain nerve cells

Creators

  • 1. Stanojevic, I.
  • 2. 'Vinca' Institute of Nuclear Science, P.O.Box 522, Belgrade, (Serbia)

Description

Purinergic signaling is composed of three modulatory components: a) source of extracellular nucleotides, b) specific receptor expression for these transmitter molecules and c) ectonucleotidase selection that dictate cell response gradually degradation extracellular nucleotides to nucleosides. ATP acts as a fast excitatory transmitter in the CNS. Postsynaptic actions of ATP are mediated by an extended family of purinergic, P2X receptors, widely expressed throughout the CNS. NTPDases hydrolyse extracellular ATP and ADP to AMP and are responsive for purinergfic termination. To investigate if ionizing irradiation could modulate CNS purinergic signalization we monitored activity of NTPDases and abundance of P2X7 receptor in synaptic plasma membranes after whole-body acute irradiation using low (0,5Gy) or therapeutic (2Gy) doses, 1h i 72h after irradiating juvenile (15-day old) and adult (90-day old) rats. Acute irradiation modulate purinergic system components investigated at the different ways in the rat development brain SPM and in the adult brain dependent of dose and time after irradiation

Abstract (Serbian)

Purinergicka signalizacija sadrzi tri modulatorne komponente: a) izvor vancelijskih nukleotida, b) specificne membranske receptore za ove transmiterske molekule i c) ektonukleotidaze koje diktiraju celijski odgovor postepenom degradacijom vancelijskih nukleotida do nukleozida. Adenozin trifosfat (ATP) je brzi ekscitatorni neurotransmiter u centralnom nervnom sistemu (CNS-u). Postsinapticko delovanje ATP-a posredovano je familijom specificnih purinergickih receptora, P2X, koji su siroko eksprimirani u CNS-u. Membranski enzimi nukleotid trifosfodifosfohidrolaze (NTPDaze) hidrolizuju vancelijske nukleotide, ATP i ADP do AMP i odgovorni su za terminaciju purinergicke signalizacije. Da bi ispitali da li jonizujuce zracenje moze da modulise purinergicku signalizaciju u CNS, pracena je promena aktivnosti NTPDaza i kolicina receptora P2X7 u plazma membranama nervnih zavrsetaka nakon akutnog ozracivanja celog tela malom (0,5Gy) ili teraeutskom (2Gy) dozom 1h i 72h nakon zracenja kod pacova u juvenilnom (15-dana stare zivotinje) i adultnom (90-dana stare zivotinje) uzrastu. Akutno zracenje celog tela modulise ispitivane komponente purinergickog sistema u mozgu pacova i nacin modulacije zavisi od stepena razvica CNS-a, primenjene doze zracenja i vremena nakon ozracivanja

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Part of:
Radiation Protection Society of Serbia and Montenegro. Proceedings of 24. Symposium

Additional details

Additional titles

Original title (Serbian)
Efekat jonizujuceg zracenja na modulaciju purinergicke signalizacije u nervnim celijama mozga pacova

Publishing Information

Publisher
Institute of Nuclear Science VINCA, Radiation Protection Society of Serbia and Montenegro
Imprint Place
Belgrade (Serbia)
ISBN
978-86-7306-089-7
Imprint Title
Radiation Protection Society of Serbia and Montenegro. Proceedings of 24. Symposium
Imprint Pagination
440 p.
Journal Page Range
p. 273-276
Report number
INIS-RS--1134

Conference

Title
24. Symposium of Radiation Protection Society of Serbia and Montenegro
Dates
3-5 Oct 2007
Place
Zlatibor (Serbia)

INIS

Country of Publication
Serbia
Country of Input or Organization
Serbia
INIS RN
39070120
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Conference, Numerical Data
Descriptors DEI
APOPTOSIS; ATP; BIOLOGICAL RADIATION EFFECTS; BRAIN; CENTRAL NERVOUS SYSTEM; DOSE-RESPONSE RELATIONSHIPS; EXPERIMENTAL DATA; HYDROLYSIS; IONIZING RADIATIONS; MODULATION; NERVE CELLS; NUCLEOSIDES; NUCLEOTIDES; PURINES; RADIATION DOSES; RATS; RECEPTORS; SIGNALS
Descriptors DEC
ANIMAL CELLS; ANIMALS; AROMATICS; AZAARENES; BIOLOGICAL EFFECTS; BODY; CENTRAL NERVOUS SYSTEM; CHEMICAL REACTIONS; DATA; DECOMPOSITION; DOSES; HETEROCYCLIC COMPOUNDS; INFORMATION; LYSIS; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; NUCLEOTIDES; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PROTEINS; RADIATION EFFECTS; RADIATIONS; RIBOSIDES; RODENTS; SOLVOLYSIS; SOMATIC CELLS; VERTEBRATES

Optional Information

Notes
5 refs., 2 figs. This record replaces record 38114316 Imprint:Zbornik radova sa 24. Simpozijuma Drustva za zastitu od zracenja Srbije i Crne Gore