Published October 1975 | Version v1
Journal article Metadata-only

An enzymic method for the determination of [1-14C] lactose

Creators

  • 1. Trinity Coll., Dublin (Ireland)

Description

A simple, rapid and specific method for the determination of [1-14C] lactose in biological fluids is described. It is based on the enzymic removal of the 1-14C atom of lactose as [14C] carbon dioxide, using commercially available enzymes. The assay involves only one critical addition and the entire reaction can be carried out in a scintillation vial. (author)

Additional details

Identifiers

DOI
10.1039/an9750000758;

Publishing Information

Journal Title
The Analyst
Journal Volume
100
Journal Issue
1195
Series
Analyst.
Journal Page Range
758
ISSN
0003-2654

INIS

Country of Publication
United Kingdom
Country of Input or Organization
United Kingdom
INIS RN
7223160
Subject category
S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Descriptors DEI
BIOLOGICAL MATERIALS; CARBON 14; ENZYMES; HYDROLYSIS; LABELLED COMPOUNDS; LACTOSE; QUALITATIVE CHEMICAL ANALYSIS; QUANTITATIVE CHEMICAL ANALYSIS; TRACER TECHNIQUES
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CARBON ISOTOPES; CHEMICAL ANALYSIS; CHEMICAL REACTIONS; DECOMPOSITION; DISACCHARIDES; EVEN-EVEN NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; NUCLEI; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; RADIOACTIVE MATERIALS; RADIOISOTOPES; SACCHARIDES; SOLVOLYSIS; YEARS LIVING RADIOISOTOPES

Optional Information

Notes
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