Evaluation the regeneration effect of Liang-Xue-Yu-Chang decoction on intestinal epithelium of rats with radiation enteritis

Objective: To study the effects of Liang-Xue-Yu-Chang decoction on intestinal epithelium of rats with radiation enteritis. Methods: Sixty male SD rats were divided into three groups randomly: normal rats as control, abdominal radiation control group given distilled water after 10 Gy radiation, and abdominal radiation plus drug group given Liang-Xue-Yu-Chang decoction after 10 Gy radiation. Drugs or distilled water were chronically administered to animals for 7 days. After that, 5-bromodexyridine (BrdU) was injected into abdominal cavity, SP immunohistochemistry was used to evaluate intestinal epithelium proliferating cell nuclear antigen (PCNA) and the positive cells labeled with Ki67 and BrdU. Results: The number of intestinal epithelium cells with PCNA expression, positive Ki67 and BrdU were 22.0±2.7, 71.2±5.7, and 26.2±5.9 respectively in the drug treatment group, significantly higher than those in the abdominal radiation control group (11.2±1.9, 61.6±5.5, and 11.3±2.2)(t = 14.629, 5.420, 11.582, P < 0.05), but lower than those in the normal control group (30.4±5.7, 86.6±5.1, and 32.3±3.2)(t = 14.291, 9.004, 4.731, P < 0.05). Conclusion: Liang-Xue-Yu-Chang decoction could improve the proliferation of intestinal epithelium in the rats with radiation enteritis. (authors)