Early diagnosis of fertility of the dihaploid from the phenotype of the ancestral haploid in tobacco

The method of haploidization, ans subsequent dihaploidization, was applied for the production of isogenic tobacco (Nicotiana tabacum L.) lines. Under the conditions of the experiment, a total of 22 months elapsed from the seeding of the anthers of the original haploid cultivar (Kabak-Koulak Porroion 14/a) to the production of G2 dihaploid seeds. Foliage and inflorescence characters of the haploid and G1 dihaploid plants were used for the early diagnosis of fertility of the G2 dihaploid plants. (author)